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At the core of the ICEPlex System is STAR technology, allowing the user to perform high multiplex assays with a broad dynamic range. The patented STAR technology is essentially the marriage of PCR and Capillary Electrophoresis. At the heart of the STAR technology is the ability to electrokinetically inject molecules from PCR into the capillaries and run electrophoresis is real-time.
STAR technology provides a quantitative answer and allows for mixed target type reactions, or multi-modal assays. Because the ICEPlex instrument has the ability to identify and follow amplicons by size during the reaction, there is no need to use more than two dyes and there is no need for excessive peak deconvolution. Using the ICEPlex System we have separate 53 amplicons using just one dye! Theoretically, it is possible to run fully quantitative 100-plex reactions with the ICEPlex System.
Three easy automated steps to high multiplex qPCR using STAR technology:
- End-labeled PCR
- Injection to Capillary Electrophoresis
- Analysis providing a report of copy numbers (or Ct)